HCT-116 Cas9 Stable Cell Line

AcceGen’s HCT-116 Cas9 Stable Cell Line is a Stable Cell Line derived from HCT-116 cells through lentiviral transfection with the bioengineered Cas9 protein gene. These cells continuously and robustly express Cas9 protein over an extended period. Using this cell line, researchers can efficiently achieve gene knockout, knock-in, and point mutations by transfecting sgRNA alone or co-transfecting both sgRNA and donor DNA. Additionally, HCT-116 Cas9 Stable Cell Line can be employed to create a cell knockout library, providing a simple solution to the challenges of Cas9 transfection.