H1975
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H1975, also known as H-1975 or NCI-H1975, is an epithelial cell line derived in 1988 from the lung tissue of a non-smoking female afflicted with non-small cell lung cancer. Notably, it carries a mutated p53 gene and expresses the dual mutations L858R/T790M in EGFR. This cell line exhibits low CD82 expression levels, which can be increased through treatment with Trichostatin (TsA). H1975 cells are typically cultured as a monolayer and require subculturing by splitting confluent cells at a ratio of 1:2 to 1:4, two to three times per week for optimal maintenance and propagation.
Why choose H1975 from AcceGen?
The H1975 cell line is characterized by its exceptional viability and quality, maintained through diligent daily monitoring to guarantee the absence of bacterial, yeast, and fungal contaminants. Furthermore, rigorous PCR-based analysis has confirmed the absence of mycoplasma, HIV, HBV, and HCV, ensuring the integrity of the cell line. Identity is also confirmed via STR analysis, contributing to the reliability and consistency of this valuable research tool.
| Product Code | H1975; H-1975; NCIH1975 |
| Species | Human |
| Cat.No | ABC-TC521S |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Epithelial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Lung |
| Disease | Adenocarinoma |
| Storage | Liquid Nitrogen |
| Product Type | Human Lung Cancer Cell Lines |
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
FOR RESEARCH USE ONLY
The H1975 cell line plays a crucial role in various applications, particularly in immuno-oncology and lung cancer research. It serves as a valuable tool for conducting preclinical tests related to lung cancer treatment, enabling researchers to investigate the impact of mutated epidermal growth factor receptor (EGFR) and its response to treatments. Moreover, H1975 cells have been instrumental in addressing T790-mediated resistance to EGFR tyrosine kinase inhibitors and aiding in the development of strategies to overcome this resistance. Additionally, they are employed in 3D cell culture models and xenograft models to further our understanding of lung cancer and potential therapeutic interventions.
