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Transfected Stable Cell Lines

Human Oct4 EGFP Reporter Cell Line-H1

  • BSL

    1

  • 42
GFP expression linked directly to the Oct4 promoter. A GFP Neomycin construct was added to the H1 cell line via homologous recombination and targeted to the POUF1 gene. Cells will express GFP when Oct4 is expressed (e.g. undifferentiated state). Oct4/GFP positive cells are selectable using Neomycin. These cells tend to differentiate more frequently and are […]
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GFP expression linked directly to the Oct4 promoter. A GFP Neomycin construct was added to the H1 cell line via homologous recombination and targeted to the POUF1 gene. Cells will express GFP when Oct4 is expressed (e.g. undifferentiated state). Oct4/GFP positive cells are selectable using Neomycin. These cells tend to differentiate more frequently and are best grown in slightly sparser conditions. It is not unusual to have to manually remove differentiation at each passage in the absence of selection.

Species

Human

Cat.No

ABC-RC232D

Product Category Transfected Stable Cell Lines
Size/Quantity

1 vial

Shipping Info

Dry Ice

Growth Conditions

37 ℃, 5% CO2

Biosafety Level

1

Storage

Liquid Nitrogen

Product Type

Reporter Stable Cell Line

Host Cell

H1

Citation

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Application

  • For research use only

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To succeed in cell culture
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