Glucagon-like peptide-1 receptor (GLP-1R) is a receptor protein found on pancreatic cells and neurons in the brain. In humans, it is synthesized by the GLP1R gene located on chromosome 6. It is a member of the glucagon-like peptide receptor family in G protein-coupled receptors.Activated GLP-1R stimulates the adenylyl cyclase pathway, leading to increased insulin synthesis and release. Therefore, GLP-1R has been a target for developing diabetes treatments (commonly referred to as GLP1R agonists). GLP-1R is also expressed in the brain, involved in appetite control.GLP-1R is a multifunctional G protein-coupled receptor that recognizes specific ligands at the N-terminus and modulates cellular pathways by coupling to various G proteins (Gαs, Gαi, Gαo, and Gαq/11). Upon binding with GLP-1, G protein α subunits dissociate from β and γ subunits, Gαs protein couples, activates adenylyl cyclase (cAMP), increases cellular cAMP levels, elevates protein kinase A (PKA) content, activates downstream signaling pathways, and increases insulin gene transcription.GLP1R Reporter CHO-K1 Cell Line is a luciferase reporter cell line constructed based on the cAMP-PKA signaling pathway. When GLP-1 binds to its receptor, the subunits dissociate. Gαs protein couples, activates adenylyl cyclase, increases cellular cAMP levels, and enhances PKA content. Activation of downstream signaling pathways leads to the expression of luciferase. Luciferase readings represent the activation effect of the signaling pathway, making it suitable for evaluating the in vitro effects of GLP1R-related drugs.
