GCGR Reporter HEK-293 Cell Line

Glucagon Receptor (GCGR) is a 62 kDa protein activated by glucagon, belonging to the B class of G protein-coupled receptor family. GCGR is mainly expressed in the liver and kidneys. Upon activation by glucagon, GCGR binds with heterotrimeric Gs (αβγ), inducing adenylyl cyclase activation, increasing cellular cAMP levels. cAMP activates PKA, leading to phosphorylation of transcriptional gene regulatory proteins and their translocation into the nucleus.GCGR Reporter HEK-293 Cell Line is a luciferase reporter cell line constructed based on the GCGR signaling pathway. When glucagon binds with its receptor GCGR, the complex activates adenylyl cyclase, elevating cellular cAMP levels. cAMP then activates PKA, phosphorylating transcriptional gene regulatory proteins, thereby activating luciferase expression. Luciferase readings represent the activation effect of the signaling pathway, making it useful for evaluating the in vitro effects of GCGR-related drugs.