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F9 | ||||
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Product Name | F9 | |||
Price | Get Quote | |||
Cat.No | ABC-TC0247 | Species | Mouse-Male | |
Size/Quantity | 1 vial | Biosafety Level | 1 | |
Shipping Info | Dry Ice | Storage | Liquid Nitrogen | |
Description | F9 cells can be stimulated to differentiate into parietal endoderm in the presence of retinoic acid and dibutyryl cyclic AMP (cAMP); Differentiating cells synthesize plasminogen activator, laminin and type IV collagen; CAMP is active only on cells that have been treated with retinoic acid; Tested and found negative for ectromelia virus (mousepox); Culture vessels must be coated with 0.1% Gelatin prior to use. To do so, cover the surface to of the vessel with 0.1% Gelatin (Difco) in sterile distilled water for 2 hours at 4C, then wash three times with sterile distilled water. Treated flasks and dishes can be stored at room temperatureTumorigenecity: Isoenzyme: Histopathology: teratoma, testicular; Embryonal carcinomaSubculture: Split ratio: Media change: Reverse transcritase: Productionplasminogen activator; Laminin; Type IV collagen. | |||
Source Organ | Embryo | |||
Recommended Medium And Supplement | DMEM + 10% FBS | |||
Citation Guide | When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID). | |||
Application | For research use only | |||
Growth Conditions | 37 ℃, 5% CO2 | |||
Growth Mode | Adherent | |||
Product Type | Mouse Teratocarcinoma Cell Lines | |||
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