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F9

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Product Name

F9

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Cat.No

ABC-TC0247

Species

Mouse-Male

Size/Quantity

1 vial

Biosafety Level

1

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

F9 cells can be stimulated to differentiate into parietal endoderm in the presence of retinoic acid and dibutyryl cyclic AMP (cAMP); Differentiating cells synthesize plasminogen activator, laminin and type IV collagen; CAMP is active only on cells that have been treated with retinoic acid; Tested and found negative for ectromelia virus (mousepox); Culture vessels must be coated with 0.1% Gelatin prior to use. To do so, cover the surface to of the vessel with 0.1% Gelatin (Difco) in sterile distilled water for 2 hours at 4C, then wash three times with sterile distilled water. Treated flasks and dishes can be stored at room temperatureTumorigenecity: Isoenzyme: Histopathology: teratoma, testicular; Embryonal carcinomaSubculture: Split ratio: Media change: Reverse transcritase: Productionplasminogen activator; Laminin; Type IV collagen.

Source Organ

Embryo

Recommended Medium And Supplement
Citation Guide

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Application

For research use only

Growth Conditions

37 ℃, 5% CO2

Growth Mode

Adherent

Product Type

Mouse Teratocarcinoma Cell Lines

Product Image AcceGen Frozen Cells & Cell Lines 1 vial
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