COLO-668
1
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Human lung oat cell carcinoma. Derived from a brain metastasis of a 47 year old female with oat cell carcinoma of the lung.
| Product Code | Colo 668; COLO668; Colo668; Colorado 668 |
| Species | Human |
| Cat.No | ABC-TC0160 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Epithelial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Lung |
| Disease | Lung Small Cell Cancer |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Lung Cell Lines |
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For research use only
COLO-668 cells were derived from a metastatic site of a human colorectal adenocarcinoma. They are widely used in cancer research, particularly in studies related to colorectal cancer biology, drug testing, and molecular studies.
The recommended seeding density for COLO-668 cells can vary depending on the specific experimental requirements and growth characteristics of the cells. As a general guideline:
Initial Seeding Density: COLO-668 cells are typically seeded at a density ranging from 1 × 10^5 to 5 × 10^5 cells per milliliter (cells/mL) of medium.
Adherence Considerations: COLO-668 cells are epithelial in origin and generally adhere well to tissue culture vessels. However, certain conditions or issues may affect their adherence:
Cell Health: Ensure that the cells are healthy and not undergoing stress due to factors like incorrect medium composition, pH imbalance, or excessive handling.
Surface Coating: COLO-668 cells may require specific surface coatings (e.g., collagen, fibronectin) to promote adherence. Check if the culture vessel has been properly coated before seeding.
Cell Density: If seeded at too low a density, cells might not adhere well. Try increasing the seeding density slightly or adjusting the incubation time after seeding to allow for attachment.
Cell Passage Number: Higher passage number cells may have reduced adherence properties. Consider using cells from earlier passages if adherence remains an issue.
Medium Composition: Ensure the medium is appropriate for COLO-668 cells and contains necessary supplements for their growth and adherence.
By addressing these factors, you can optimize the conditions for COLO-668 cell culture and promote adherence to the culture vessel surface, ensuring robust growth and experimental success.
