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Canine Brain Microvascular Endothelial Cells | ||||
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| Product Name | Canine Brain Microvascular Endothelial Cells | |||
| Price | Get Quote | |||
| Cat.No | ABC-H0075X | Species | Canine | |
| Size/Quantity | 1 vial | Biosafety Level | 1 | |
| Shipping Info | Dry Ice | Storage | Liquid Nitrogen | |
| Description | Canine Brain Microvascular Endothelial Cells are isolated from brain of beagle dog. Each vial contains at least 0.5×10^6 cells per ml and are delivered frozen. Canine Brain Microvascular Endothelial Cells are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:2 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended. | |||
| Disease | Normal | |||
| Source Organ | Brain | |||
| Quality Control | All cells test negative for mycoplasma, bacteria, yeast, and fungi. | |||
| Recommended Medium And Supplement | ABM-H0075X Canine Brain Microvascular Endothelial Cells Medium Kit | |||
| Citation Guide | When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID). | |||
| Application | For research use only | |||
| Growth Conditions | 37 ℃, 5% CO2 | |||
| Cell Type | Endothelial | |||
| Growth Mode | Adherent | |||
| Product Type | Canine Primary Cells | |||
| Product Image |  | |||
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Frequently Asked Questions
What are Canine Brain Microvascular Endothelial Cells and what role do they play?
Canine Brain Microvascular Endothelial Cells are specialized cells derived from the microvasculature of the canine brain. They play a crucial role in forming the blood-brain barrier (BBB) and are essential for studying canine neurovascular health and disease.
What methods are used to isolate and purify Canine Brain Microvascular Endothelial Cells
These cells are isolated from the brain microvasculature of dogs through a series of enzymatic digestion and mechanical dissociation steps. Purification typically involves selective adherence and cell sorting techniques to ensure a high-purity population of endothelial cells.
What research applications are Canine Brain Microvascular Endothelial Cells used for?
These cells are primarily used in research focusing on the blood-brain barrier, neuroinflammation, neurodegenerative diseases, and drug delivery to the brain. They provide a valuable in vitro model for studying the interaction between brain endothelial cells and various pathogens or therapeutic agents.
What are the optimal culture conditions for Canine Brain Microvascular Endothelial Cells?
Canine Brain Microvascular Endothelial Cells should be cultured in a specialized endothelial cell growth medium, typically supplemented with essential growth factors, hormones, and serum. They require a controlled environment at 37°C with 5% CO2 and 95% humidity.
What role do Canine Brain Microvascular Endothelial Cells play in neurological research?
These cells are instrumental in studying the structural and functional properties of the blood-brain barrier. They help researchers understand how the BBB regulates the passage of substances between the bloodstream and the brain, and how its dysfunction can contribute to neurological disorders.
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