Caco-2

Caco-2 is a human epithelial cell line derived from colon carcinoma. It exhibits characteristics similar to normal enterocytes when grown on plastic dishes or nitrocellulose filters. Caco-2 cells have the ability to undergo differentiation and form a monolayer of cells that exhibit numerous characteristics commonly seen in absorptive enterocytes, including the presence of a brush border layer resembling that of the small intestine. Over time, these cells show increased in alkaline phosphatase activity in the culture, highlighting their epithelial cell nature. Notably, Caco-2 cells possess a specific genetic mutation (heterozygous G-to-C missense transversion) in codon 245 of exon 5. This mutation results in a substitution of glycine to alanine, potentially leading to impaired target recognition by protein kinase enzymes and altered accessibility for phosphorylation at that site.

Why choose Caco-2 from AcceGen?

Caco-2 cells from AcceGen exhibit several notable features. Firstly, they are sterile and negative for bacteria, fungi, and mycoplasma contamination. Secondly, these cells maintain high viability, ensuring reliable and consistent experimental results. Additionally, Caco-2 cells are produced using professional production techniques, ensuring quality and consistency across batches. These features make Caco-2 cells a reliable and trustworthy model for studying various aspects of intestinal physiology and drug absorption.