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C57BL/6 Mouse Retinal Microvascular Endothelial Cells

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C57BL/6 Mouse Retinal Microvascular Endothelial Cells from AcceGen are isolated from tissue of pathogen-free laboratory mice. Mouse Retinal Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen’s Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells […]
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Home | Primary Cells | Animal Primary Cells | Mouse Primary cells | C57BL/6 Mouse Retinal Microvascular Endothelial Cells

C57BL/6 Mouse Retinal Microvascular Endothelial Cells from AcceGen are isolated from tissue of pathogen-free laboratory mice. Mouse Retinal Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen’s Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×10^6 cells per ml and are delivered frozen.
Mouse Retinal Microvascular Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Species

C57BL/6 Mouse
Cat.No

ABC-TC3241
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Product Category Primary Cells
Size/Quantity

1 vial
Cell Type

Endothelial
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Retina
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Mouse Primary Cells

Citation

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AcceGen Frozen Cells & Cell Lines 1 vial
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Application

  • For research use only

Frequently Asked Questions

  • What is C57BL/6 Mouse Retinal Microvascular Endothelial Cells?

    C57BL/6 Mouse Retinal Microvascular Endothelial Cells are isolated from retinal microvasculature, characterized by barrier function and angiogenesis, and specifically express VE-cadherin and CD31.

  • Application of C57BL/6 Mouse Retinal Microvascular Endothelial Cells

    C57BL/6 Mouse Retinal Microvascular Endothelial Cells are used in research to study retinal vascular biology, blood-retinal barrier function, and angiogenesis. They are vital for understanding diabetic retinopathy, age-related macular degeneration, and other retinal diseases. These cells enable the evaluation of new therapeutic agents and the exploration of molecular pathways involved in retinal vascular health. Their application in developing treatments for retinal disorders holds significant promise for advancing ocular disease management and therapy.

  • What are the cell culture conditions for C57BL/6 Mouse Retinal Microvascular Endothelial Cells?

    C57BL/6 Mouse Retinal Microvascular Endothelial Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by AcceGen.

  • What happens to C57BL/6 Mouse Retinal Microvascular Endothelial Cells during in vitro culture?

    C57BL/6 Mouse Retinal Microvascular Endothelial Cells initially adhere to the culture surface as single cells or small clusters, exhibiting a spindle-shaped morphology. As they proliferate, they form a monolayer with a cobblestone appearance, resembling typical endothelial cell morphology. Over time, endothelial cells may display elongated or polygonal shapes depending on their confluence and passage number. Additionally, they may develop tube-like structures when cultured in appropriate angiogenic conditions, mimicking their in vivo behavior.

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Tags

  • Endothelial (424)
  • Kidney (147)
  • Mouse (639)
  • Normal (1578)

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