C57BL/6 Mouse Neurons-substantia nigra

Frequently Asked Questions

• What is C57BL/6 Mouse Neurons-substantia nigra?

  The central nervous system is composed of neurons and glia. Neurons, which act as the anatomic, functional, and trophic units, are dynamically polarized to transmit electrochemical signals. Despite their diverse sizes and shapes, neurons possess essential morphological features critical for complex communication. The substantia nigra, a midbrain cluster of dopaminergic neurons, is involved in learning, addiction, and movement. Dysfunction or loss of neurons-substantia nigra is linked to disorders such as drug addiction, schizophrenia, and Parkinson’s disease.

• Application of C57BL/6 Mouse Neurons-substantia nigra

  C57BL/6 Mouse Neurons-substantia nigra are essential in experimental research for studying Parkinson’s disease, drug addiction, and schizophrenia. These neurons provide a reliable model to investigate the pathophysiology of dopaminergic neuron degeneration and dysfunction. Additionally, they are crucial for understanding the mechanisms of synaptic plasticity, neurotransmitter release, and receptor signaling pathways in the substantia nigra, contributing to the development of targeted treatments for various neurodegenerative and psychiatric disorders.

• Morphology of C57BL/6 Mouse Neurons-substantia nigra

  When culturing C57BL/6 mouse substantia nigra neurons in vitro, they undergo significant morphological changes. In the initial phase (0-3 days), neurons appear round, gradually adhere to the substrate, and extend short neurites. In the intermediate phase (4-7 days), neurites lengthen and branch, forming preliminary dendritic and axonal networks with developing synaptic connections. In the mature phase (8-14 days and beyond), neurons form complex neural networks, further extending and branching dendrites and axons, with increased synaptic connections and displaying dopaminergic characteristics such as dopamine synthesis and release. During culture, ensure sterile techniques, timely medium changes, constant temperature and gas environment (37oC, 5% CO2), and control cell density to prevent overcrowding or sparse growth.