C57BL/6 Mouse Epidermal Keratinocytes

C57BL/6 Mouse Primary Epidermal Keratinocytes from AcceGen are isolated from skin tissue of pathogen-free laboratory mice. C57BL/6 Mouse Primary Epidermal Keratinocytes are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in AcceGen’s Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5×106 cells per ml and is delivered frozen. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by AcceGen. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.