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Product Code | MBMMAC |
Species | C57BL/6 Mouse |
Cat.No | ABC-TC3171 |
Quality Control | All cells test negative for mycoplasma, bacteria, yeast, and fungi. |
Product Category | Primary Cells |
Size/Quantity | 1 vial |
Cell Type | Macrophage |
Shipping Info | Dry Ice |
Growth Conditions | 37 ℃, 5% CO2 |
Source Organ | Bone Marrow |
Disease | Normal |
Biosafety Level | 1 |
Storage | Liquid Nitrogen |
Product Type | Mouse Primary Cells |
Mouse Bone Marrow Macrophages are derived from the tibias and femurs of pathogen-free laboratory adult mice. Mouse Bone Marrow Macrophages are grown in 100 mm treated tissue culture dish and incubated in AcceGen’s Culture Medium for 7-10 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 6×10^6 cells and is delivered frozen.
Mouse Bone Marrow Macrophages are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended.
Mouse Bone Marrow Macrophages are tested for expression of markers using antibodies, CD11b by flow cytometry.
Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
For research use only