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BV-2

965

Product Name

BV-2

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Product Code

BV2

Cat.No

ABC-TC212S

Species

Mouse-C57BL/6

Size/Quantity

1 vial

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

BV-2 Cells Derivation and biomarker
BV-2 is a type of microglial cell derived from C57/BL6 murine. The BV2 cells are immortalized by v-raf/v-myc carrying J2 retrovirus. BV2 express nuclear v-myc and the cytoplasmic v-raf oncogene products as well as the env gp70 antigen at the surface level. BV2 microglia cell line retains microglia morphological and functional characteristics. In accordance with v-raf/v-myc expressed characters, the metabolic and proliferation rate of in vitro BV-2 greatly exceeds that of other microglia.

Why Choose BV-2 from AcceGen?
AcceGen BV-2 cells are positive for MAC1 and MAC2 antigens, negative for MAC3, glial fibrillary acidic protein (GFAP) and galactocerebroside (GC).

Source Organ

Brain

Citation Guide

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
Hagan, N., Kane, J.L., Grover, D., Woodworth, L., Madore, C., Saleh, J., Sancho, J., Liu, J., Li, Y., Proto, J. and Zelic, M., 2020. CSF1R signaling is a regulator of pathogenesis in progressive MS. Cell death & disease, 11(10), pp.1-25.
Lee, H.J., Seo, M., Baek, M., Shin, Y.P., Lee, J.H., Kim, I.W., Hwang, J.S. and Kim, M., 2020. Inhibitory Effect of Protaetiamycine 6 on Neuroinflammation in LPS-stimulated BV-2 Microglia. Journal of Life Science, 30(12), pp.1078-1084.
Inada, M., Xu, H., Takeuchi, M., Ito, M. and Chen, M., 2021. Microglia increase tight-junction permeability in coordination with Müller cells under hypoxic condition in an in vitro model of inner blood-retinal barrier. Experimental Eye Research, 205, p.108490.
Liu, Y., Hammel, G., Shi, M., Cheng, Z., Zivkovic, S., Wang, X., Xu, P., He, X., Guo, B., Ren, Y. and Zuo, L., 2021. Myelin debris stimulates NG2/CSPG4 expression in bone marrow-derived macrophages in the injured spinal cord. Frontiers in Cellular Neuroscience, 15, p.80.
Li, J., Cheng, X., Fu, D., Liang, Y., Chen, C., Deng, W. and He, L., 2022. Autophagy of Spinal Microglia Affects the Activation of Microglia through the PI3K/AKT/mTOR Signaling Pathway. Neuroscience, 482, pp.77-86.

Martin, N. P., & Harry, G. J. (2022). Imaging inflammasome activation in microglia. Current Protocols, 2, e578. doi: 10.1002/cpz1.578.
Lee, J.H., Ji, S.H., Lim, J.S. et al. Anti-neuroinflammatory Effects and Brain Pharmacokinetic Properties of Selonsertib, an Apoptosis signal-regulating Kinase 1 Inhibitor, in mice. Neurochem Res 47, 3829–3837 (2022). https://doi.org/10.1007/s11064-022-03777-9.

Hasriadi et al. “Antinociceptive efficacy of Clerodendrum petasites S. Moore, a Thai medicinal plant, and its CNS safety profiles.” Journal of traditional and complementary medicine vol. 13,1 81-92. 12 Nov. 2022, doi:10.1016/j.jtcme.2022.11.001.

Lim, Jae Sung, et al. “In Vitro Anti-Inflammatory Effects of Symplocos Sumuntia Buch.-Ham. Ex D. Don Extract via Blockage of the NF-ΚB/JNK Signaling Pathways in LPS-Activated Microglial Cells.” Plants, vol. 11, no. 22, Nov. 2022, p. 3095. Crossref, https://doi.org/10.3390/plants11223095.

Janpaijit, Sakawrat et al. “Anti-neuroinflammatory effects of Cleistocalyx nervosum var. paniala berry-seed extract in BV-2 microglial cells via inhibition of MAPKs/NF-κB signaling pathway.” Heliyon vol. 8,11 e11869. 28 Nov. 2022, doi:10.1016/j.heliyon.2022.e11869.

Application

For research use only
BV-2 Cells Application and example model
In the central nervous systems (CNS), microglial cells are resident macrophages. BV-2 shares properties with body macrophages with respect to the antigen profile, their phagocytic capacity and antimicrobial activity. Like other microglia, BV-2 can be activated to release pro-inflammatory cytokines by oxidative stress or inflammatory factors. Such stimulus possibly triggers neurodegenerative disorder such as Alzheimer and Parkinson disease, thus BV-2 is widely used as an alternative model system for primary microglia and neurodegenerative disease model in vitro. The model is usually induced by Lipopolysaccharide (LPS) treatment.

Key Features

-Backed by AcceGen advanced technology
-Cryopreserved for highest viability and plating efficiency
-Quality-tested for accurate results

Growth Conditions

37 ℃, 5% CO2

Cell Type

Microglial

Product Type

Mouse Brain Cancer Cell Lines

Product Image

AcceGen Frozen Cells & Cell Lines

AcceGen product bv-2 cell line

Frequently Asked Questions

  • What are BV-2 cells, and why should I use them in my research?

    BV-2 cells are a murine microglial cell line commonly used as a model for studying microglial function and neuroinflammation. They offer a consistent and reproducible system for investigating the immune responses of microglia in the central nervous system.

  • How can I optimize the culture conditions for BV-2 cells?

    Medium: Use high-glucose DMEM supplemented with 10% fetal bovine serum (FBS), L-glutamine, and antibiotics (penicillin-streptomycin).

     

    Incubation: Maintain cells at 37°C in a humidified atmosphere with 5% CO₂.

     

    Medium Renewal: Change the medium every 2-3 days to ensure optimal nutrient availability and waste removal.

  • What specific growth factors or supplements can enhance the activation of BV-2 cells?

    Lipopolysaccharide (LPS): Often used to activate microglial cells and induce an inflammatory response.

     

    Interferon-gamma (IFN-γ): Enhances the activation and pro-inflammatory response of microglia.

     

    Tumor Necrosis Factor-alpha (TNF-α): Used to study inflammatory signaling pathways.

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Reviews of BV-2
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One thought on “BV-2”


Date: 10-20-2022 15:38:03


Not only was the sales rep awesome, but the cells arrived right on time. They were packaged nicely into a box with dry ice and are exactly what we wanted.


Quote No.: AGK4005 Name: Coleen Thompson

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