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Species | Bovine |
Cat.No | ABC-TC025N |
Quality Control | All cells test negative for bacteria, yeast, fungi, and mycoplasma. |
Product Category | Primary Cells |
Size/Quantity | 1 vial |
Cell Type | Epithelial |
Shipping Info | Dry Ice |
Growth Conditions | 37 ℃, 5% CO2 |
Source Organ | Rumen |
Disease | Normal |
Storage | Liquid Nitrogen |
Product Type | Bovine Primary Cells |
The rumen is the first stomach of ruminant animals and serves as a crucial digestive organ in these animals. The morphological structure and function of the rumen epithelium play a significant role in maintaining digestive system stability and promoting animal health.
The rumen wall in ruminant animals is composed of three layers, progressing from the outer to inner layers: the serous membrane layer, the muscularis layer, and the mucosa layer. Within the rumen wall, the rumen epithelium is closely associated with its absorptive and metabolic functions.
AcceGen’s Bovine Rumen Epithelial Cells are derived from normal rumen tissue in experimental animals. Each vial contains 5×10^5 cells with a volume of 1 ml. Positive immunofluorescent staining for broad-spectrum cytokeratin (PCK) or cytokeratin-19 (CK-19) confirms cell purity exceeding 90%. The cells are free from HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
For research use only
The rumen is a crucial organ in the digestive system of ruminant animals, responsible for fermenting cellulose, providing nutrients, producing volatile fatty acids for absorption and utilization by the animal. Additionally, the rumen regulates microbial communities, digests food, and maintains internal environmental stability.
Bovine Rumen Epithelial Cells, essential for ruminant digestion, are widely applied in research fields such as ruminant nutrition, microbiology, and gastrointestinal health. Researchers use them to explore nutrient absorption mechanisms, investigate the rumen microbiome’s impact on digestion, and assess the epithelial barrier function, aiding in the development of livestock feed additives and strategies for improving ruminant health.
When passaging bovine rumen epithelial cells, the doubling time typically ranges between 24 to 48 hours, depending on various factors such as culture conditions and cell density. During passaging, it’s crucial to maintain an appropriate cell density, typically around 70-80% confluence, to ensure optimal growth and viability. This ensures that the cells remain healthy and maintain their physiological characteristics for downstream experiments and applications.