Technical Support
[email protected]
Hot Products
- Human Orbital Fibroblasts
- Human Microglia
- Human Pulmonary Alveolar Epithelial Cells
- Human Colonic Fibroblasts
- Human Type II Alveolar Epithelial Cells
- Human Valvular Interstitial Cells
- Human Thyroid Epithelial Cells
- C57BL/6 Mouse Dermal Fibroblasts
- Human Alveolar Macrophages
- Human Dermal Fibroblasts, Adult
- Human Lung Fibroblasts, Adult
- Human Retinal Muller Cells
- Human Articular Chondrocytes
- Human Retinal Pigment Epithelial Cells
- Human Pancreatic Islets of Langerhans Cells
- Human Kidney Podocyte Cells
- Human Renal Proximal Tubule Cells
-
Can non-proliferating cells be sub-cultured?
Non-proliferating cells cannot be sub-cultured because they do not proliferate. This includes the following cell types: neurons, microglia, macrophages, and some other cell types. See the instructions for details.
-
Do we perform human leukocyte antigen typing for cells?
If you have specific requirements, please contact our customer service department before ordering.
-
How do I choose a primary cell culture environment?
We recommend using 37 ℃, 5% CO2 incubator to culture all primary cells of AcceGen.
-
Can I refreeze AcceGen primary cells?
We do NOT recommend customers to refreeze AcceGen primary cells.
-
As for blood collection, what is the difference between K2EDTA, Sodium Citrate and Sodium Heparin?
The tubes may contain additional substances called anticoagulants, which can be helpful for better preservation of the blood for further processing in the medical laboratory. The choice of different anticoagulants depends upon the downstream medical/biochemical analysis being undertaken.
Heparin is a highly charged biological molecule-polymeric glucosaminoglycan. It prevents coagulation of blood but can be co-purified with the nucleic acids depending upon the preparation method used. It is a well-known inhibitor of PCR and preferably should be avoided if collecting blood for PCR analysis.
EDTA can be used for whole blood hematology determinations and immunohematology testing like ABO grouping, Rh typing, antibody screening.
Citrate can be used in different types of blood bank studies that include HLA phenotyping, DNA and paternity testing.
-
Do you have any primary epithelial cells from main organs like lung, liver, colon, or kidney that are HLA-A2 positive? If not, what other primary cells that are NOT immune cells you have that are HLA-A2 positive?
We do not have primary epithelial cells from organs. Currently, only immune cells and PBMCs have HLA information.
-
Would you be able to provide some additional information on tight junction protein expression- E-cadherin, ZO-1 or Claudin 3 in human intestinal epithelial cells? How long do these cells take to differentiate and form tight junction proteins?
Epithelial Cells grow in the medium form a monolayer of polarized epithelial cells with tight junction formation as evidenced by ZO-1 (tight junction marker) staining.
ZO-1 staining is typically performed on day 3 or 4. A time course of expression has not performed.
-
Comparing to primary cells directly originate from organs, are there any differences in iPSC derived primary cells?
iPSCs can be differentiated to functional and specific cells in vitro with demonstrable in vitro and in vivo research. Genetic modifications employed at the iPSC level can deliver desirable immunotherapeutic attributes to the generated effectors.
-
Can iPSC cells be grown without a feeder layer? If a feeder layer is required are you using MEF cells?
iPSCs usually grow best when attached to other cells or an extracellular matrix and grow traditionally in cultures with feeder layers.
MEFs are fairly universally used. If using MEFs as a feeder layer, the MEF media must be removed before the iPS cells can be plated. The iPS cells can be cultured in MEF dishes conditioned with iPS medium. We recommend conditioning new feeder dishes prior to passaging iPS cells into them.
-
What types of coating solutions does AcceGen usually provide?
We offer various coating solutions for primary cell culture, including:
- CS420Poly-L-Lysine Solution- READY TO USE
- CS425Gelatin-Based Coating Solution- READY TO USE
- CS430Bovine Plasma Fibronectin
- CS431Alpha Coating Solution
- CS432Q-coating Solution
- CS433Xeno-free Coating Solution
For each type of primary cell, we have carefully selected and confirmed the most suitable coating solution. In addition, we also offer CS434 iPSC Coating Solution for certain iPSC lines to cater to diverse research needs.
