FAQs

    1.     Remove a vial of cells from liquid nitrogen, taking care to protect your hands and eyes.    
    2.     Loosen the cap on the vial for 1/4 second to release the liquid nitrogen that may be trapped in the threads, then re-tighten the cap.    
    3.     Place the frozen vial in a 37 ℃ water bath. Gently hold and rotate the vial until the contents are completely melted. The vial was immediately removed from the water bath, wiped clean with 70% ethanol, and then transferred to a sterile area.    
    4.     Carefully remove the cover without touching the internal threads. Gently resuspend the contents of the vial and dispense into a balanced poly-L-lysine or fibronectin-coated culture vessel containing the culture medium (see specific instructions listed on the product table).    
    5.     Close the lid or lid of the culture dish and gently shake the culture dish to evenly distribute the cells. If necessary, loosen the cover for gas exchange.    
    6.     Return the petri dish to the incubator (37 ℃, 5% CO2 / 95% air).    
        For best results, do NOT disturb the culture for at least 16 hours after starting the culture. The medium was renewed the next day to remove residual DMSO and unattached cells (unless stated otherwise on the product table).
    Note: For detailed instructions, see the cell manual.        

We do NOT recommend centrifuging the cells to remove DMSO residue.

    1.     When the cells are thawed in a water bath, the nozzle of the cryopreservation tube should be higher than the water surface to prevent the liquid contaminating the cells.    
    2.     Water baths are prone to bacterial growth, and water should be disinfected regularly to avoid contaminating cells.    
    3.     When using cell culture dishes, they must be handled aseptically to prevent cell contamination.        

We recommend adding 5 ml of medium to the T-25 flask, 15 ml of medium to the T-75 flask, and 30 ml of medium to the T-150 flask.

For specific instructions, see the product table for the type of battery used. Generally, the medium should be changed every 2-3 days depending on the confluence of cells.

It depends on the cell type, refer to the manual for details.

Cells do show weak adhesion as compared to most other adherent cell lines in our hands. Please take great care when you trypsinize cells or replace the medium. Normally, you can use diluted trypsin (e.g. 0.01%), which is sufficient to detach cells.

Primary Cells – Cells derived directly from human or animal tissue. And primary cells usually have a limited lifespan. Compared to cell lines, primary cells maintain a lot of characters and markers seen with the donors.

Cell Lines – Cells have undergone a genetic transformation that has been continually passaged over a long period of time. Cell lines are finite or continuous. Usually, a cell line can be sub-cultured for 30-80 passages. Compared to primary cells, cell lines have lost the true characteristics of the original tissue from which they were isolated but are preferably used for convenience as they are easy to handle and widely published.

The specific shelf life will be marked on the medium bottle, and the shelf life of the kit form is generally longer. The shelf life of complete medium is shorter.

For example, cryopreserved human gastric epithelial cells are viable for at least 2 years when stored under these conditions.