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Hot Products
- Human Orbital Fibroblasts
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- Human Type II Alveolar Epithelial Cells
- Human Valvular Interstitial Cells
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- C57BL/6 Mouse Dermal Fibroblasts
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- Human Dermal Fibroblasts, Adult
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- Human Retinal Muller Cells
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What is a stem cell?
Stem Cells, also calls Progenitor Cells, are a kind of self-replicating pluripotent cells, which can differentiate into multiple functional cells under certain conditions. Given this characteristic, some diseases can be cured by transplanting stem cells or related derivatives into patients to replace damaged cells.
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Can I use your stem cells for curing disease?
Unfortunately, stem cells provided by AcceGen are only for research use. We would suggest you to seek help from professional hospital or therapeutic center.
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How many times can stem cells be passaged?
Times would vary from different types of stem cells. For example, pluripotent stem cells which are derived from the inner cell mass of blastocysts or induced by transcription factors from somatic cells, hPSC can self-renew indefinitely. If you would like to know the exact passage times of your stem cells, please consult our customer time.
Theoretically, pluripotent stem cells have an infinite lifespan. Due to the culture condition restriction, the more time cells passaged, the higher risk of chromosomal differentiation or instability. The karyotype analysis is recommended every 10 passages.
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Can I expand or cryopreserve hPSC-derived cardiomyocytes?
We would not suggest you to expand or re-freeze hPSC-derived cardiomyocytes in any circumstances.
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What is the difference between embryonic stem cells, adult stem cells, and umbilical cord stem cells?
Embryonic Stem Cells are a type of cells isolated from early embryos or primitive gonads. They have the characteristics of infinite proliferation, self-renewal, and multi-directional differentiation. Whether in vitro or in vivo, ES cells can be induced to differentiate into almost all cell types in the body.
Adult Stem Cells are undifferentiated cells that exist in a differentiated tissue that can self-renew and specialize to form the cells that make up this type of tissue. Adult stem cells are found in various tissues and organs of the body
Umbilical cord stem cells are isolated from the umbilical cord of newborn infants and are less mature than adult stem cells. Umbilical cord stem cells are a type of somatic stem cell.
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Can iPSC cells be grown without a feeder layer? If a feeder layer is required are you using MEF cells?
iPSCs usually grow best when attached to other cells or an extracellular matrix and grow traditionally in cultures with feeder layers.
MEFs are fairly universally used. If using MEFs as a feeder layer, the MEF media must be removed before the iPS cells can be plated. The iPS cells can be cultured in MEF dishes conditioned with iPS medium. We recommend conditioning new feeder dishes prior to passaging iPS cells into them.
