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What is the minimum quantity you can provide for miRNA Agomir/Antagomir service?
The minimum quantity is 2 OD (1 OD corresponds to 33 μg). Please note we have significant bulk order discounts.
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What kinds of labels you can do for miRNA Agomir/Antagomir service?
We can usually label the miRNA Agomir/Antagomir with fluorescent tag, such as FAM, CY3, etc.
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How to convert the OD and nmol of RNA oligo?
microRNA agomirs are double-stranded, while microRNA antagomirs are single-stranded. The amount of materials are quantified in OD and both agomirs/antagomirs are synthesized to yield the same OD. In general, there is a simple calculation as follows.
Agomir: 1 OD duplex=2.5 nmol = 33µg; Antagomir: 1 OD simplex=5 nmol = 33µg.
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What should be provided for microRNA agomir/ antagomir synthesis?
To synthesize microRNA agomir/antagomir, you just need to provide the accession number of the mature microRNA and the quantity you need to synthesize.
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Can the negative control (NC) of agomir and antagomir be used universally? Does NC have to be used?
It’s not universal, because agomir is a double-stranded RNA and antagomir is a single-stranded RNA. It is recommended to use NC as a control group to exclude false positive results.
Click the picture or title to enter the corresponding page to browse AcceGen MicroRNA Agomir/Antagomir products:
– Human MicroRNA Agomir/Antagomir
– Mouse MicroRNA Agomir/Antagomir
– Rat MicroRNA Agomir/AntagomirFor more detailed or not mentioned technical questions, contact us at +1-862-248-1155 or [email protected], our customer service department is always here to support you.