Technical Support
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Hot Products
- In-Stock Tumor Cell Lines
- Human Orbital Fibroblasts
- Human Microglia
- Human Pulmonary Alveolar Epithelial Cells
- Human Colonic Fibroblasts
- Human Type II Alveolar Epithelial Cells
- Human Valvular Interstitial Cells
- Human Thyroid Epithelial Cells
- C57BL/6 Mouse Dermal Fibroblasts
- Human Alveolar Macrophages
- Human Dermal Fibroblasts, Adult
- Human Lung Fibroblasts, Adult
- Human Retinal Muller Cells
- Human Articular Chondrocytes
- Human Retinal Pigment Epithelial Cells
- Human Pancreatic Islets of Langerhans Cells
- Human Kidney Podocyte Cells
- Human Renal Proximal Tubule Cells
- Will my primary cell vial be 100% pure?
Primary cells are never 100% pure, but we try to make the cells as pure as possible.
- What passage number would endothelial primary cells be?
You will receive endothelial cells from passages 2 or 3, depending on cell purity.
- How many passages can a normal primary cell take?
It depends on the cell type, refer to the manual for details. Primary endothelial cells can be expanded 3-7 passages by the split ratio of 1:2 under the cell culture conditions specified by AcceGen.
- Can non-proliferating cells be sub-cultured?
Non-proliferating cells cannot be sub-cultured because they do not proliferate. This includes the following cell types: neurons, microglia, macrophages, and some other cell types. See the instructions for details.
- Do we perform human leukocyte antigen typing for cells?
If you have specific requirements, please contact our customer service department before ordering.
- How do I choose a primary cell culture environment?
We recommend using 37 ℃, 5% CO2 incubator to culture all primary cells of AcceGen.
- Can I refreeze AcceGen primary cells?
We do NOT recommend customers to refreeze AcceGen primary cells.
- As for blood collection, what is the difference between K2EDTA, Sodium Citrate and Sodium Heparin?
The tubes may contain additional substances called anticoagulants, which can be helpful for better preservation of the blood for further processing in the medical laboratory. The choice of different anticoagulants depends upon the downstream medical/biochemical analysis being undertaken.
Heparin is a highly charged biological molecule-polymeric glucosaminoglycan. It prevents coagulation of blood but can be co-purified with the nucleic acids depending upon the preparation method used. It is a well-known inhibitor of PCR and preferably should be avoided if collecting blood for PCR analysis.
EDTA can be used for whole blood hematology determinations and immunohematology testing like ABO grouping, Rh typing, antibody screening.
Citrate can be used in different types of blood bank studies that include HLA phenotyping, DNA and paternity testing.
- Do you have any primary epithelial cells from main organs like lung, liver, colon, or kidney that are HLA-A2 positive? If not, what other primary cells that are NOT immune cells you have that are HLA-A2 positive?
We do not have primary epithelial cells from organs. Currently, only immune cells and PBMCs have HLA information.
- Would you be able to provide some additional information on tight junction protein expression- E-cadherin, ZO-1 or Claudin 3 in human intestinal epithelial cells? How long do these cells take to differentiate and form tight junction proteins?
Epithelial Cells grow in the medium form a monolayer of polarized epithelial cells with tight junction formation as evidenced by ZO-1 (tight junction marker) staining.
ZO-1 staining is typically performed on day 3 or 4. A time course of expression has not performed.
