Luciferase Expressing Cells

Luciferases are a diverse group of oxidative enzymes that play a pivotal role in bioluminescence by catalyzing reactions that produce light. These enzymes are naturally found in a wide range of organisms, from bacteria and fungi to insects. However, in mammalian systems, luciferases from fireflies, click beetles, and various marine organisms, including the sea pansy (Renilla luciferase), copepod (Gaussia luciferase), and deep-sea shrimp (NanoLuc), have been adapted as reporter enzymes due to their unique properties.

The primary advantage of luciferase as a reporter is its ability to detect extremely low levels of gene expression. Unlike fluorescent reporters, luciferase-based bioluminescence is virtually free of background noise, enabling highly sensitive measurements. Typically, luciferase expression is quantified by adding luciferin and ATP to cell lysates, followed by luminometric analysis of the emitted light. Despite its advantages, the luciferase system does have some limitations, including the challenge of delivering luciferin to intact cells and the consumption of ATP, which is essential for cellular metabolism.

At AcceGen, we offer a variety of Luciferase Expressing Cells to support and enhance your scientific research. Explore our product offerings below, and if you have specific requirements, such as custom cell development, please visit our Custom Cell Service or contact us directly at inquiry@accegen.com.