KARPAS 1718
1
Discover top-quality products tailored for scientific and medical research. Request a personalized quote today
to enhance your projects.
Human splenic lymphoma. KARPAS 1718 is derived from the peripheral blood of a patient diagnosed with transformed splenic lymphoma with villous lymphocytes.
| Product Code | KARPAS-1718; Karpas 1718; KARPAS 1718; K1718 |
| Species | Human |
| Cat.No | ABC-TC0490 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Lymphoblast |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Disease | Lymphoma |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Lymphoma Cell Lines |
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
For research use only
Cultivating KARPAS 1718 cells, a human lymphoma cell line, can present several challenges. Here are some common problems and strategies to address them:
Common Problems and Solutions
1. Poor Cell Viability
Problem: Cells show low viability after thawing or during culture.
Solution: Ensure gentle handling during thawing. Quickly thaw the vial in a 37°C water bath and immediately transfer to pre-warmed complete medium. Use fresh, high-quality FBS and other supplements. Maintain optimal incubation conditions (37°C, 5% CO2) and avoid temperature fluctuations.
2. Slow Growth or Stagnation
Problem: Cells grow slowly or stop proliferating.
Solution: Ensure the medium is fresh and contains all necessary supplements. KARPAS 1718 cells typically require RPMI-1640 medium supplemented with 10-20% FBS and 1% Penicillin-Streptomycin. Check for proper cell density; excessively high or low densities can affect growth. Ideally, maintain cell density between 2 x 10^5 to 1 x 10^6 cells/mL. Perform regular medium changes to provide fresh nutrients and remove waste.
3. Contamination
Problem: Bacterial, fungal, or mycoplasma contamination.
Solution: Practice strict aseptic techniques, including working in a sterile biosafety cabinet. Use antibiotics cautiously and consider regular mycoplasma testing. If contamination is detected, discard the contaminated culture and thoroughly disinfect the work area.
For KARPAS 1718 cells, the medium should typically be changed every 2-3 days. This ensures that the cells have a consistent supply of nutrients and that waste products do not accumulate to toxic levels. However, the exact frequency may vary based on the cell density, growth rate, and specific conditions of your culture system. Regular monitoring of the cells and the medium is recommended to adjust the feeding schedule as needed.
The recommended seeding density for KARPAS 1718 cells can vary depending on the specific experimental setup and the growth characteristics of the cells. Here are some general guidelines:
Initial Seeding Density: Typically, KARPAS 1718 cells are seeded at a density ranging from 1 × 10^5 to 5 × 10^5 cells per milliliter (cells/mL) of medium.
Experimental Needs: The exact seeding density can be adjusted based on the experimental requirements, such as the growth rate needed, the time frame of the experiment, and the desired cell density at harvest.
Confluence: For routine maintenance and passage, cells are often seeded to reach about 70-80% confluence at the time of subculture or experimental treatment to ensure healthy growth and avoid over-confluence.
Optimization: It’s advisable to perform optimization experiments to determine the ideal seeding density for your specific experimental conditions, as cell lines can vary in their growth characteristics and requirements.
If you have specific experimental goals or constraints, adjusting the seeding density accordingly can help optimize cell growth and experimental outcomes.
