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B95-8

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Product Name

B95-8

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Product Code

B95.8; B 95.8; B 95-8; B-95-8; B958

Cat.No

ABC-TC0065

Species

Saguinus oedipus

Size/Quantity

1 vial

Biosafety Level

2

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

The B95-8 cell line is derived from Cotton-top Tamarin Monkey peripheral blood lymphocyte, and its name comes from the B95 strain of Epstein Barr Virus (EBV), which was isolated from a case of transfusion-induced mononucleosis and used to immortalize monkey blood leukocytes. These cells release high titers of transforming EBV and should be handled at laboratory containment level 2. Originally thought to be derived from marmosets, DNA profiling has revealed that the B95-8 cell line comes from Cotton-top Tamarin Monkey Peripheral Blood Lymphocytes. The cells have a predominantly suspension-like morphology, with ovular cells or small clumps, and around 10% of the cells are adherent. They can be cultured in RPMI1640 medium supplemented with 10% FBS, with cell detachment sometimes achieved using 0.25% trypsin or trypsin/EDTA.

 

Why choose B95-8 from AcceGen?

B95-8 cells from AcceGen are prepared in sterile conditions by professional operators within a professional laboratory setting. These cells undergo rigorous quality control measures to ensure their high viability. Stringent protocols are followed during the preparation and handling to maintain sterility and minimize contamination risks. The use of professional operators and adherence to strict quality control standards guarantee the reliability and consistency of B95-8 cells, making them a trusted resource for research and experimentation purposes.

Source Organ

Blood

Recommended Medium And Supplement
Citation Guide

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Application

FOR RESEARCH USE ONLY

The B95-8 cell line is extensively employed for its role as a source of Epstein Barr Virus (EBV) in transforming and immortalizing human B lymphocytes into continuous cell lines. This enables the indefinite propagation of cells from specific donors, ensuring a continuous supply of genomic material without the need for resampling.

 

These cells have diverse applications, including studying the effects of cytokines on B cell growth by regulating key cell surface molecules involved in intercellular communication. They are valuable for investigating the response to B-cell growth and differentiation factors. The versatility of the B95-8 cell line makes it a valuable tool for understanding the mechanisms underlying B cell biology and intercellular signaling.

Growth Conditions

37 ℃, 5% CO2

Cell Type

Fibroblast

Growth Mode

Suspension

Product Type

Monkey Lymphocytes Cell Lines

Product Image

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