F9
1
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F9 cells can be stimulated to differentiate into parietal endoderm in the presence of retinoic acid and dibutyryl cyclic AMP (cAMP); Differentiating cells synthesize plasminogen activator, laminin and type IV collagen; CAMP is active only on cells that have been treated with retinoic acid; Tested and found negative for ectromelia virus (mousepox); Culture vessels must be coated with 0.1% Gelatin prior to use. To do so, cover the surface to of the vessel with 0.1% Gelatin (Difco) in sterile distilled water for 2 hours at 4C, then wash three times with sterile distilled water. Treated flasks and dishes can be stored at room temperatureTumorigenecity: Isoenzyme: Histopathology: teratoma, testicular; Embryonal carcinomaSubculture: Split ratio: Media change: Reverse transcritase: Productionplasminogen activator; Laminin; Type IV collagen.
| Species | Mouse-Male |
| Cat.No | ABC-TC0247 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Embryo |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Mouse Teratocarcinoma Cell Lines |
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