EL4.NOB-1
1
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EL4.NOB-1, derived from EL4.BU.OU6, is a specialized murine C57BL/6N ascites lymphoma lymphoblast cell line meticulously selected for its elevated expression of IL-1 receptors. This unique cellular model exhibits distinctive characteristics, notably displaying resistance to TGF-β-induced growth arrest while retaining sensitivity to autoinduction and the suppression of IL-1-induced IL-2 production.
In coculture experiments involving IL-1β, EL4.NOB-1 cells demonstrate a growth-promoting response to TGF-β, a phenomenon effectively counteracted by the introduction of the phosphatidylcholine-dependent phospholipase C (PC-PLC) inhibitor, D609. Additional investigations have been conducted utilizing specific enzyme inhibitors targeting protein kinases (PK) C and A, mitogen-activated protein kinase (MAPK), phospholipase A2 (PLA2), phosphatidylinositol-dependent (PI)-PLC, and PC-PLC.
Deliberately engineered to express a substantial quantity of IL-1 receptors, EL4.NOB-1 facilitates the secretion of IL-2 upon IL-1 stimulation, eliminating the necessity for supplementary agents in the induction process. This cell line stands as an invaluable asset for the exploration of intricate signaling pathways associated with IL-1 receptor activation and TGF-β resistance within the context of cellular growth and cytokine production. It serves as a precise and reliable tool for researchers delving into the nuanced dynamics of immune responses and cellular signaling in biomedical studies.
Why choose EL4.NOB-1 from AcceGen?
Unlock the potential of your biomedical research with AcceGen’ s EL4.NOB-1 Cell Line – a meticulously characterized and high-performance cellular tool. Ensuring the highest standards of quality, this cell line has undergone rigorous testing, affirming its freedom from mycoplasma, bacteria, viruses, and other toxins.
Key Features:
Exceptional Purity:
EL4.NOB-1 has been thoroughly tested to guarantee its purity, providing a reliable foundation for a wide range of biomedical applications.
Vital Viability:
Prior to freezing, all cells in the EL4.NOB-1 Cell Line boast a remarkable viability rate exceeding 95%, ensuring robustness and reliability in your experiments.
| Product Code | EL4NOB1; NOB-1 |
| Species | Mouse |
| Cat.No | ABC-TC0244 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Lymphoblast |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Blood |
| Disease | Lymphoma |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Mouse Lymphoma Cell Lines |
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
FOR RESEARCH USE ONLY
Here are some key applications:
Immunology Studies
EL4.NOB-1 is valuable for investigating immune responses, particularly in understanding the signaling pathways associated with IL-1 receptors. Researchers can explore the intricate mechanisms involved in immune system modulation.
Cytokine Production Research
The cell line’ s ability to secrete IL-2 after IL-1 stimulation makes it an excellent model for studying cytokine production. This application is crucial for unraveling the molecular events underlying immune responses.
Pharmacological Assays
EL4.NOB-1 is suitable for conducting pharmacological assays. By using specific enzyme inhibitors targeting protein kinases (PK) C and A, mitogen-activated protein kinase (MAPK), phospholipase A2 (PLA2), phosphatidylinositol-dependent (PI)-PLC, and PC-PLC, researchers can gain insights into the effects of various compounds on cellular signaling pathways.
Cellular Growth and Autoinduction Studies
Researchers can utilize EL4.NOB-1 to investigate cellular growth patterns, autoinduction processes, and the suppression of IL-1-induced IL-2 production in the context of TGF-β resistance. This is valuable for understanding cell behavior under specific stimuli.
Signaling Pathway Analysis
EL4.NOB-1 can be employed to delve into signaling pathways associated with IL-1 receptor activation. This is critical for deciphering the intricate molecular events that regulate cellular responses to external stimuli.
Molecular Biology Studies
EL4.NOB-1 can be used for molecular biology studies, aiding in the characterization of genes and proteins involved in immune responses and cellular signaling.
