CTX TNA2
1
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CTX TNA2 represents a type I astrocyte cell line, derived from primary cultures of type 1 astrocytes sourced from the frontal cortex tissue of neonatal rats. Following an initial plating phase, these cultures underwent transfection with a DNA construct harboring the oncogenic early region of SV40, which was placed under the transcriptional regulation of the human GFAP promoter (pGFA-SV-Tt), along with pPGK-neo featuring the murine phosphoglycerate kinase gene promoter. As a result, CTX TNA2 cells have retained key characteristics consistent with the type 1 astrocyte phenotype. Notably, around 20% of these cells exhibit immunoreactivity for glial fibrillary acidic protein (GFAP), indicating their astrocytic identity. Additionally, they possess a high-affinity gamma-aminobutyric acid (GABA) uptake mechanism, which is susceptible to inhibition by beta-alanine. In terms of protein production, CTX TNA2 cells synthesize alpha 2 macroglobulin at levels similar to those found in primary astrocytes but exhibit markedly reduced transferrin production. Immunostaining analysis further confirms the presence of SV40 T-antigen within the nuclei of over 95% of these cells.
Why choose CTX TNA2 from AcceGen?
CTX TNA2 cells from AcceGen exhibit exceptional viability and quality, having been meticulously cultured under optimal conditions. These cells are delivered in a sterile state, rigorously screened for the absence of bacteria, yeast, mycoplasma, and human pathogens, including HIV, HBV, and HCV. Additionally, they are cryopreserved by skilled operators and undergo stringent quality control measures to ensure their reliability and utility in various research applications.
| Species | Rattus Norvegicus |
| Cat.No | ABC-TC0199 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Fibroblast |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Brain |
| Disease | Normal |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Rat Nerve Tumors Cell Lines |
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
FOR RESEARCH USE ONLY
CTX TNA2 cell line finds significant applications in the realms of central nervous system development and biochemistry research. Moreover, when co-cultured with primary porcine brain endothelial cells, it serves as a valuable tool in the creation of an in vitro blood-brain barrier (BBB) model. This model facilitates the investigation of intricate biochemical and physiological processes related to the BBB, offering insights into essential aspects of neurobiology and neuropharmacology.
